Yang B. Plant Genome Engineering. Methods and Protocols 2023 torrent

Textbook in PDF format

This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Protocols is a valuable tool for researchers interested in learning more about this developing and important field.
Genome Engineering Systems
PAM-Less CRISPR-SpRY Genome Editing in Plants
Type I-D CRISPR System-Mediated Genome Editing in Plants
CRISPR/​LbCas12a-Mediated Genome Editing in Soybean
Base Editing in Poplar Through an Agrobacterium-Mediated Transformation Method
Genetic Engineering of Potato (Solanum tuberosum) Chloroplasts Using the Small Synthetic Plastome “Mini-Synplastome”
Design and Validation Tools
Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout Using CRISPR-BETS
Construction of CRISPR/​Cas9 Multiplex Genome Editing System in Rice
Use of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants
Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies
Transgene-Free Delivery of Machinery
Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line
Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector
Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.)
Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves
Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide
Generation and Analysis of Engineered Lines
Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method
Efficient Targeted Mutagenesis in Brassica Crops Using CRISPR/Cas Systems
Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.)
A CRISPR/​Cas9 Protocol for Target Gene Editing in Barley
Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration
Stepwise Optimization of Real-Time RT-PCR Analysis
CRISPR/​Cas9 Technology for Potato Functional Genomics and Breeding
Genome Engineering for Crop Improvement
Recent Advances in Engineering of In Vivo Haploid Induction Systems