Baldari C. The Immune Synapse. Methods and Protocols 2ed 2023 torrent

Textbook in PDF format

This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as methods related to sites of action and immunotherapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, The Immune Synapse: Methods and Protocols, Second Edition serves as an ideal practical guide for researchers working in this dynamic field.
Measuring the Co-Localization and Dynamics of Mobile Proteins in Live Cells Undergoing Signaling Responses
Fluorescence-Based Measurements of Two-Dimensional Affinity in Membrane Interfaces
Surfaces for Study of Receptor Dynamics on T Cells
Molecular Dynamics Simulations of Immune Receptors and Ligands
Investigating Diffusion Dynamics and Interactions with Scanning Fluorescence Correlation Spectroscopy (sFCS)
Combined FRET-FLIM and NAD(P)H FLIM to Analyze B Cell Receptor Signaling Induced Metabolic Activity of Germinal Center B Cells In Vivo
Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP)
Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy
En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses
High- and Super-Resolution Imaging of Cell-Cell Interfaces
Separation of Single Core and Multicore Lytic Granules by Subcellular Fractionation and Immunoisolation
Microvillar Cartography:​ A Super-Resolution Single-Molecule Imaging Method to Map the Positions of Membrane Proteins with Respect to Cellular Surface Topography
T Cell Immunological Synaptosomes:​ Definition and Isolation
Dynamics of Immune Cell Microvilli
Visualization of Myddosome Assembly in Live Cells
Detection of Telomere Transfer at Immunological Synapse
Bottom-Up Assembly of Bioinspired, Fully Synthetic Extracellular Vesicles
A DNA Origami-Based Biointerface to Interrogate the Spatial Requirements for Sensitized T-Cell Antigen Recognition
Leveraging DNA Origami to Study Phagocytosis
Fabrication of Nanoscale Arrays to Study the Effect of Ligand Arrangement on Inhibitory Signaling in NK Cells
Measurement of Forces for Trans-Endocytosis at Dorsal and Ventral Sides of the Cell
Functionalized Lipid Droplets and Microfluidics Approach to Study Immune Cell Polarity In Vitro
Quantifying Immune Cell Force Generation Using Traction Force Microscopy
Characterizing Biophysical Parameters of Single TCR-pMHC Interactions Using Optical Tweezers
Isolation of the B Cell Immune Synapse for Proteomic Analysis
Analyzing Single Cell Secretions by “Shadow Imaging”
Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
Interactions of Tissue-Resident T Cells
Live Imaging of CAR T Cell Ca2+ Signals in Tumor Slices Using Confocal Microscopy
Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse
In Vitro Generation of Human Tolerogenic Monocyte-Derived Dendritic Cells
Methods of Machine Learning-Based Chimeric Antigen Receptor Immunological Synapse Quality Quantification
Imaging CAR-T Synapse as a Quality Control for CAR Engineering