Oatley J. Spermatogonial Stem Cells. Methods and Protocols 2023 torrent

Textbook in PDF format

This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls.Cutting-edge and comprehensive, Spermatogonial Stem Cells: Methods and Protocols is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.
Introduction:​ The Why’s and How’s for Studying Spermatogenesis and Spermatogonial Stem Cells
Optimization of Testicular Fixation-Embedding Techniques for Improved Evaluation of Mammalian Spermatogonial Morphology and Function
Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes
Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia
Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell—Part 1:​ Epigenomics Assays
Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell:​ Part 2—Computational Analysis of Epigenomics Data
Primary Cultures of Spermatogonia and Testis Cells
Reconstitution of Human Prospermatogonia​l Development from Human-Induced Pluripotent Stem Cells
RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia
Isolation of Undifferentiated​ Spermatogonia from Adult and Developing Mouse Testes
Spermatogonial Transplantation
Viral Transduction of Mammalian Spermatogonial Stem Cells
Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis
Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments
Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches
Lineage Tracing of Spermatogonial Stem Cells Within the Male Germline
Perspectives:​ Approaches for Studying Livestock Spermatogonia
Perspectives:​ Methods for Evaluating Primate Spermatogonial Stem Cells